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The Best PDF Creator & Editor 2020 to create and edit PDF files on Windows. PDF is the most popular and powerful application in the world of.The MIT License (MIT)
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An improved method for the extraction of G protein-coupled receptors.
The expression, purification, and characterization of G protein-coupled receptors (GPCR) is a well-established practice in the pharmaceutical industry. This process commonly involves the isolation of the protein with the use of detergents, which is laborious and may lead to low yields. In order to streamline this process, a rapid and efficient extraction technique was developed. The method involves the use of three major steps. The first consists of the denaturation of the membrane proteins using a range of buffers, the second consists of the removal of interfering lipids via the addition of detergents, and the third consists of the affinity purification using a ligand coupled to a solid support. In the present protocol, purified GPCRs are obtained with a purity suitable for crystallographic and functional studies. Moreover, the method can
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